In-stem labelling allows visualization of DNA strand displacements by distinct fluorescent colour change.

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Nuclear organization and gene expression: visualization of transcription and higher order chromatin structure Authors

The way chromatin is organized inside the nucleus of eukaryotic cells is a key factor in gene control. It seems that the cell is able to transcriptionally regulate genomic loci by changing their local environment in the nucleus. Such environment may inhibit, permit or boost gene activity. To unravel underlying molecular mechanisms it is essential to develop techniques that allow the simultaneou...

Labelling of live cells using fluorescent aptamers: binding reversal with DNA nucleases

A reversible cell labelling method has been developed for non-destructive and non-invasive cell labelling and purification. Our method uses high affinity single strand DNA (ssDNA) aptamers against surface exposed target molecules on cells. The aptamers are subsequently removed from the cell surface using DNase nuclease treatment. We exemplified our method by labelling human acute lymphoblastic ...

Immuno-gold Labelling of Chlamydia trachomatis

Background Chlamydia trachomatis is considered as an important cause of preventable sexually transmitted diseases worldwide. It is known to be of an obligate intracellular nature and enters its target cells via an endocytic process. As major outer membrane protein (MOMP) is one of the main candidates for the attachment and entry of chlamydia to the host cells we have tried to label the epitopes...

Transient expression of green fluorescent protein in radish (Raphanus sativus) using a turnip mosaic virus based vector

It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...